Signalling

Part:BBa_J23120:Design

Designed by: Will Bosworth   Group: iGEM06_Berkeley   (2006-10-08)


Locked RFP (J23077) with OriT (J01003). Lock that can be transferred via conjugation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1088
    Illegal NgoMIV site found at 1098
    Illegal AgeI site found at 675
    Illegal AgeI site found at 787
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 990


Design Notes

see construction file on http://openwetware.org/wiki/IGEM:UC_Berkeley/2006 for actual construction method.


Source

.

References